Factors that affect symbiotic nitrogen fixation in the soil 

• Presence of nitrogen fixing bacteria (of the genus Rhizobium)
• Presence of root legume roots in whose root nodule bacteria live
• Presence favorable temperature
• Presence of oxygen in the soil for respiration of bacteria
• Presence of nitrogen in the soil to be fixed
• Presence of zinc in the soil: zinc is necessary for nitrogen fixation acting as a secondary signal for nitrogen fixation
• Presence of antimicrobial agents in the soil.

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Dr. Bbosa Science