Testing for non-reducing sugars

Testing for non-reducing sugars

Testing for non-reducing sugars

1.When boiled with Benedict’s solution or Fehling’s’ solution, the color remains blue.

2.When boiled with HCl, the solution cooled, neutralized by NaOH, boiled Benedict’s or Fehling’s solution, the color changes from green, to yellow to orange.

 

HCl hydrolyses non-reducing sugars

NaOH neutralizes the excess acid because Benedict’s or Fehling’s solution does not work in acidic medium.

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